
Calcium-free Modified Barth’s Solution (CMBS) is used for defolliculation of Xenopus laevis oocytes by collagenase treatment.
Ready to Use CMBS solution or 10x concentrate available
autoclaved
Instructions for use:
Ready to use:
Direct use
10x concentrate:
For 1000 ml CMBS Solution:
1. Pour 100 ml CMBS (10x) into a flask
2. Add deionized water to a final volume of 1000 ml
Adjust to pH 7.4
Use immediately once opened. Store at 4 – 8 °C
Ional composition of CMBS Solution
NaCl = 88 mM
KCl = 1 mM
MgSO4 = 0,8 mM
TRIS-HCl = 5 mM
NaHCO3 = 2,4 mM
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Please find all required information for Ecocyte Bioscience Cell & Tissue Media like Safety Data Sheets and literature.
HS-BSCS
(High Sucrose Brain Slice Cutting Solution)
[Szabadics and Soltesz, J. Neurosci. 29(13), 4239-4251, 2009]
Download Safety Data Sheet for HS-BSCS
SHS-BSCS
(Sodium-free High Sucrose Brain Slice Cutting Solution)
[Aghajanian and Rasmussen, Synapse Vol3(4), 331-338, 1989]
Download Safety Data Sheet for SHS-BSCS
CC-BSCS
(Choline Chloride Brain Slice Cutting Solution)
[Grunditz, J. Neurosci. 28(50) 13457-13466, 2008]
Download Safety Data Sheet for CC-BSCS
MH-BSCS
(Modified HEPES Brain Slice Cutting Solution)
[Jonathan Ting www.brainslicemethods.com]
Download Safety Data Sheet for MH-BSCS
NH-BSRS
(NMDG-HEPES Brain Slice Recovery Solution)
[Jonathan Ting www.brainslicemethods.com]
Download Safety Data Sheet for NH-BSRS
ACSF
(Artificial cerebrospinal fluid)
Download Safety Data Sheet for ACSF – Ready to use
Download Safety Data Sheet for ACSF – 20x concentrate
MTS
(Modified Tyrode’s Solution)
[Himmel et al., Brit. J. Pharmacol. 166(1), 276-296, 2012]
Download Safety Data Sheet for MTS – Ready to use
Download Safety Data Sheet for MTS – 20x concentrate
NFR
(Normal Frog Ringer)
Download Safety Data Sheet for NFR – Ready to use
Download Safety Data Sheet for NFR – 10x concentrate
MBS
(Modified Barth’s Solution)
Download Safety Data Sheet for MBS – Ready to use
Download Safety Data Sheet for MBS – 10x concentrate
CMBS
(Calcium-free Barth’s Solution)
Download Safety Data Sheet for CMBS – Ready to use
Download Safety Data Sheet for CMBS – 10x concentrate
PBS
(Phosphate Buffered Solution)
Download Safety Data Sheet for PBS – Ready to use